Outline of the workshop

Opening Session
Mr. Apichart Noenplab, Agricultural Research Officer, Rice Department and Dr. Thawatchai Onjun, Executive Director, Thailand Institute of Nuclear Technology (TINT) delivered welcoming remarks. Then Mr. Obata Ryoji, Deputy Director, International Nuclear and Fusion Energy Affairs Division, Research and Development Bureau, Ministry of Education, Culture, Sports, Science and Technology of Japan gave an opening speech.

Overview Lectures
Dr. Tamada Masao reviewed FNCA activities in 2020-2022 and major success results achieved. Dr. Hase Yoshihiro, FNCA Mutation Breeding Project Leader of Japan briefly described major outcomes of the FNCA Mutation Breeding Project and the major issues to be discussed in the workshop.

Open Seminar
Open seminar on "Application of Radiation Technology and Mutation Breeding for Sustainable Agriculture" was held in the afternoon of February 21st. Firstly, Mr. Noenplab presented the topic on improving submergence tolerance in Thai rice through electron beam induced mutation for sustainable agriculture. Dr. Vichai Puripunyavanich, Thailand Institute of Nuclear Technology introduced success story and future challenge on mutation breeding in Thailand. Dr. Katsura Keisuke, Associate Professor, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, talked on the development of high-throughput phenotyping technology for efficient mutation breeding in developing countries. Dr. Sobri Bin Hussein, Research Officer, Malaysian Nuclear Agency reported success story and future challenge on mutation breeding in rice, in Malaysia. Dr. Bayarsukh Noov, Director General, Institute of Plant and Agricultural Science of Mongolia introduced success story and future challenge on mutation breeding in wheat and barley, in Mongolia.

Country Report on Mutation Breeding of Major Crops for Low-input Sustainable Agriculture under Climate Change
Ten member countries presented progress and activity plan on the Mutation Breeding of Major Crops for Low-input Sustainable Agriculture under Climate Change.

Round Table Discussion for Mutation Breeding of Major Crops for Low-input Sustainable Agriculture under Climate Change
A lead speech was delivered by Dr. Yoshihiro Hase, setting off a roundtable discussion on the direction of research and the possibilities of further cooperation among the member countries. The importance of information exchange as well as seeking a chance to start collaboration based on this platform was emphasized. The results on whole genome sequencing of promising mutant lines obtained in Bangladesh were introduced as one of the ways to deepen collaboration. It was announced that no beam time would be provided for this project in FY2023 at QST-Takasaki, Japan, due to the steep rise in electricity prices and so on. After discussion, the participants confirmed the followings.

1. All member countries agreed that the low input sustainable agriculture is still very important issue.
2. Development of mutant lines with good tolerance to biotic and abiotic stresses, such as flash flood, drought tolerance and disease resistance, are one of the effective ways to achieve low-input sustainable agriculture.
3. Adopting new technologies such as high-throughput phenotyping, genotyping or other modern technologies is beneficial to improve the classical mutation breeding protocol. Also, exchange of mutant lines/varieties among the member countries is expected to shorten the breeding period, if the situation allows. The member countries try to explore more collaboration opportunities.
4. Several member countries recognized the effectiveness of ion beams and have an interest to use ion beams together with other mutagen. Those member countries cooperatively make the necessary effort to obtain beam time at the irradiation facility of QST-Takasaki.

Minutes and Closing Session
The minutes were discussed and agreed by all participants. It will be reported to the 23rd Coordinators Meeting to be held in June 2023 in Japan. Dr. Tamada delivered closing remarks and thanked all participants for their efforts and contribution.

Technical Visit
Participants visited Thailand Institute of Nuclear Technology in Nakornnayok on February 22nd. They visited the facility of electron beam accelerator, gamma facilities, laboratories of plant in-vitro culture, and molecular biology, with detailed explanation by the experts of TINT.

1) Outline

Opening Session
Mr. Apichart Noenplab, Agricultural Research Officer, Rice Department and Dr. Thawatchai Onjun, Executive Director, Thailand Institute of Nuclear Technology (TINT) delivered welcoming remarks. Then Mr. Obata Ryoji, Deputy Director, International Nuclear and Fusion Energy Affairs Division, Research and Development Bureau, Ministry of Education, Culture, Sports, Science and Technology of Japan gave an opening speech.

Overview Lectures
Dr. Tamada Masao reviewed FNCA activities in 2020-2022 and major success results achieved. Dr. Hase Yoshihiro, FNCA Mutation Breeding Project Leader of Japan briefly described major outcomes of the FNCA Mutation Breeding Project and the major issues to be discussed in the workshop.

Open Seminar
Open seminar on "Application of Radiation Technology and Mutation Breeding for Sustainable Agriculture" was held in the afternoon of February 21st. Firstly, Mr. Noenplab presented the topic on improving submergence tolerance in Thai rice through electron beam induced mutation for sustainable agriculture. Dr. Vichai Puripunyavanich, Thailand Institute of Nuclear Technology introduced success story and future challenge on mutation breeding in Thailand. Dr. Katsura Keisuke, Associate Professor, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, talked on the development of high-throughput phenotyping technology for efficient mutation breeding in developing countries. Dr. Sobri Bin Hussein, Research Officer, Malaysian Nuclear Agency reported success story and future challenge on mutation breeding in rice, in Malaysia. Dr. Bayarsukh Noov, Director General, Institute of Plant and Agricultural Science of Mongolia introduced success story and future challenge on mutation breeding in wheat and barley, in Mongolia.

Session 1 Country Report on Mutation Breeding of Major Crops for Low-input Sustainable Agriculture under Climate Change
Ten member countries presented progress and activity plan on the Mutation Breeding of Major Crops for Low-input Sustainable Agriculture under Climate Change. The summary for each presentation is attached as Annex 3.

Session 2 Round Table Discussion for Mutation Breeding of Major Crops for Low-input Sustainable Agriculture under Climate Change
A lead speech was delivered by Dr. Yoshihiro Hase, setting off a roundtable discussion on the direction of research and the possibilities of further cooperation among the member countries. The importance of information exchange as well as seeking a chance to start collaboration based on this platform was emphasized. The results on whole genome sequencing of promising mutant lines obtained in Bangladesh were introduced as one of the ways to deepen collaboration. It was announced that no beam time would be provided for this project in FY2023 at QST-Takasaki, Japan, due to the steep rise in electricity prices and so on. After discussion, the participants confirmed the followings.

1. All member countries agreed that the low input sustainable agriculture is still very important issue.
2. Development of mutant lines with good tolerance to biotic and abiotic stresses, such as flash flood, drought tolerance and disease resistance, are one of the effective ways to achieve low-input sustainable agriculture.
3. Adopting new technologies such as high-throughput phenotyping, genotyping or other modern technologies is beneficial to improve the classical mutation breeding protocol. Also, exchange of mutant lines/varieties among the member countries is expected to shorten the breeding period, if the situation allows. The member countries try to explore more collaboration opportunities.
4. Several member countries recognized the effectiveness of ion beams and have an interest to use ion beams together with other mutagen. Those member countries cooperatively make the necessary effort to obtain beam time at the irradiation facility of QST-Takasaki.

Session 3 Minutes and Closing Session
The minutes were discussed and agreed by all participants. It will be reported to the 23rd Coordinators Meeting to be held in June 2023 in Japan. Dr. Tamada delivered closing remarks and thanked all participants for their efforts and contribution.

2) Technical Visit
Participants visited Thailand Institute of Nuclear Technology in Nakornnayok on February 22nd. They visited the facility of electron beam accelerator, gamma facilities, laboratories of plant in-vitro culture, and molecular biology, with detailed explanation by the experts of TINT.

3) Attachments
Annex 1 Program
Annex 2 List of Participants
Annex 3 Presentation Summary for Session 1

Tuesday, February 21st, 2023 (Day 1st)

Wednesday, February 22nd, 2023 (Day 2nd)

Thursday, February 23rd, 2023 (Day 3rd)

Bangladesh
Dr. ANK MAMUN (PL)
Chief Scientific Officer and Head
Bangladesh Atomic Energy Commission (BAEC)

China
Prof. SHU Qingyao (PL)
Professor
Zhejiang University

Indonesia
Dr. Sobrizal (PL)
National Research and Innovation Agency (BRIN)

Dr. Winda PUSPITASARI
Researcher
National Research and Innovation Agency (BRIN)

Mr. Arwin
National Research and Innovation Agency (BRIN)

Ms. Yuliasti
National Research and Innovation Agency (BRIN)

Mr. Muhmmad Ikbal
National Research and Innovation Agency (BRIN)

Japan
Mr. OBATA Ryoji
Deputy Director
International Nuclear and Fusion Energy Affairs Division
Research and Development Bureau
Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan

Ms. NAKAHARA Risa
Administrative Researcher
International Nuclear and Fusion Energy Affairs Division
Research and Development Bureau
Ministry of Education
Culture, Sports, Science and Technology (MEXT)

Dr. TAMADA Masao
FNCA Advisor of Japan,
QST Associate
National Institutes for Quantum Science and Technology (QST)

Dr. HASE Yoshihiro (PL)
Senior Principal Researcher
Department of Radiation-Applied Biology Research
Takasaki Advanced Radiation Research Institute
Quantum Beam Science Research Directorate
National Institutes for Quantum Science and Technology (QST)

Dr. KATSURA Keisuke
Associate Professor
Department of International Environmental and Agricultural Science
Graduate School of Agriculture
Tokyo University of Agriculture and Technology (TUAT)

Prof. NAKAI Hirokazu
Professor Emeritus
Former Vice President
Shizuoka University

Dr. HATASHITA Masanori
Principal researcher
Research & Development Department
The Wakasa wan Energy Research Center

Mr. NOMURA Tomoyuki
International Affairs and Research Department
Nuclear Safety Research Association (NSRA)

Ms. KOIKE Aki
International Affairs and Research Department
Nuclear Safety Research Association (NSRA)

Korea
Prof. KANG Si-Yong (PL)
Associate Professor
Department of Horticulture
Kongju National University

Malaysia
Dr. Abdul Rahim Bin HARUN
Director General
Malaysian Nuclear Agency

Dr. Sobri Bin HUSSEIN (PL)
Research Officer
Malaysian Nuclear Agency

Dr. Faiz Bin AHMAD
Research Officer
Malaysian Nuclear Agency

Mongolia
Dr. Bayarsukh NOOV (PL)
Director
Institute of Plant and Agricultural Science (IPAS)

Ms. Uugantsetseg Battogtokh
Researcher
Plant Breeding Division
Institute of Plant and Agricultural Science (IPAS)

The Philippines
Mr. Fernando B. AURIGUE (PL)
Senior Science Research Specialist
Department of Science and Technology (PNRI)
Philippine Nuclear Research Institute (DOST)

Mr. Christopher C. CABUSORA
Senior Science Research Specialist
Philippine Rice Research Institute (PhilRice)

Thailand
Miss. Kulsiri Klunnuruksa
Director of Division of Rice Research and Development
Rice Department

Mr. Apichart Noenplab
Agricultural Research Officer, Senior Professional Level Acting for Rice breeding expert
Rice Department

Dr. Jirapong Jairin
Agricultural Research Officer, Senior Professional Level Acting for Biotechnology Expert
Rice Department

Dr. Payorm Cobelli
Agricultural Research Officer, Senior Professional Level Acting for Rice Protection Expert
Rice Department

Dr. Ronnachai Changsri
Agricultural Research Officer, Senior Professional Level Acting for Rice Genetic Germplasm Expert
Rice Department

Mrs. Grissana Sudtasarn
Agricultural Research Officer, Senior Professional Level
Rice Department

Ms. Orathai Jaituy
Director of Foreign Relations and Special Project Group
Rice Department

Ms. Kanthita Suangtho
Agricultural Research Officer, Senior Professional Level
Rice Department

Ms. Sirima Pansiri
Agricultural Research Officer, Senior Professional Level
Rice Department

Dr. Wanporn Khemmuk
Agricultural Research Officer, Senior Professional Level
Rice Department

Dr. Pakobkit Dungthisong
Agricultural Research Officer, Senior Professional Level
Rice Department

Dr. Fuanglada Tanachote
Agricultural Research Officer, Senior Professional Level
Rice Department

Ms. Malinee Janwan
Agricultural Research Officer, Professional Level
Rice Department

Ms. Manika Noyaiem
Agricultural Research Officer, Professional Level
Rice Department

Ms. Supannikar Pakkethati
Agricultural Research Officer, Professional Level
Rice Department

Dr. Panchita Weschasan
Agricultural Research Officer, Professional Level
Rice Department

Mr. Bhorntep Seewanna
Agricultural Research Officer, Professional Level
Rice Department

Ms.Thidamas Kumyan
Agricultural Research Officer, Practitioner Level
Rice Department

Ms. Suphalaksana Sonkhongnok
Agricultural Research Officer, Practitioner Level
Rice Department

Ms. Phattharamon Khongsomyut
Agricultural Research Officer, Practitioner Level
Rice Department

Mr. Kanoknop Klinla-or
Agricultural Research Officer, Practitioner Level
Rice Department

Miss Ploypilin Thanikkul
Agricultural Research Officer, Practitioner Level
Rice Department

Mr. Ithipong Assaranurak
Agricultural Research Officer, Practitioner Level
Rice Department

Ms. Nila Rasidee
Agricultural Research Officer, Practitioner Level
Rice Department

Mr. Pakkaphon Naksit
Agricultural Research Officer, Practitioner Level
Rice Department

Ms. Ilada Choomsang
Agricultural Research Officer, Practitioner Level
Rice Department

Ms. Wanlaya Rakkaew
Agricultural Research Officer, Practitioner Level
Rice Department

Dr. Thawatchai Onjun
Executive Director
Thailand Institute of Nuclear Technology (TINT)

Dr. Vichai Puripunyavanich
Nuclear Scientist (Expert Level)
Nuclear Technology Research and Development Center
Thailand Insttitute of Nuclear Technology

Ms. Piyanuch Orpong
Nuclear Scientist
Nuclear Technology Research and Development Center
Thailand Insttitute of Nuclear Technology

Ms. Mayuree Limtiyayotin
Nuclear Scientist
Nuclear Technology Research and Development Center
Thailand Insttitute of Nuclear Technology

Ms. Lamai Maikaew
Nuclear Scientist
Nuclear Technology Research and Development Center
Thailand Insttitute of Nuclear Technology

Ms. Chatchawan Mansaithong
International Cooperation Officer
Thailand Insttitute of Nuclear Technology

Ms. Natchaya Thipsorn
International Cooperation Officer
Thailand Insttitute of Nuclear Technology

Dr. Roppon Picha
Head of Agriculture and Food Technology Section
Thailand Insttitute of Nuclear Technology

Vietnam
Dr. LE Duc Thao (PL)
Deputy Director General
Agricultural Genetics Institute (AGI)