FNCA

Mutation Breeding workshop

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- Project Review
- Introduction of the Project Leaders
- Mutation Breeding Publication Database
 
- Mutation Breeding Manual
 
- Sorghum & Soybean
- Insect Resistance in Orchid
- Disease Resistance in Banana
- Composition or Quality in Rice
 
- Papers for Project Outcome

Workshop

FNCA 2003 WORKSHOP ON MUTATION BREEDING

FNCA 2003 Workshop on Mutation Breeding took place on September 22-26
in Manila, the Philippines.

Date: Monday, September 22 - Friday, September 26, 2003
Venue: Manila Southwoods Manor, Carmona, Cavite, Philippines and
Philippine Nuclear Research Institute, Manila, Philippines
Host Organizations: Department of Science and Technology (DOST)
Philippine Nuclear Research Institute (PNRI)
Ministry of Education, Culture, Sports, Science and Technology,
Japan (MEXT)
In Cooperation with:   Japan Atomic Industrial Forum Inc. (JAIF)
Participants: Total 24 from 8 countries, i.e. China, Indonesia, Japan, Korea,
Malaysia, the Philippines, Thailand and Vietnam



Session 1:
Presentation of Country Reports
A total of seven papers on the current status of “Techniques on Reproductive Pattern in Root Crops” were presented.

Session 2: Invited Paper

A paper on “Root Crops R & D Status and Directions” was presented by the representative of PCARRD, Philippines.

Session 3: Drought Tolerance in Sorghum and Soybean Project
Country Reports were presented by participating countries (China, Indonesia for Sorghum, Philippines and Viet Nam for Soybean).

In this Project, breeding materials of sorghum and soybean have been exchanged between the respective participating countries and results from early stage of research were reported.

The utilization of ion-beam for plant breeding for development method of increasing plant genetic variability by induction of mutation was proposed. The Office of Coordinator of Japan was requested to facilitate a system of research collaboration by bridging the gap among participating countries.

Session 4: Insect Resistance in Orchid Project

Two clones from Thailand and one clone from Malaysia were exchanged at the Workshop. Indonesia will send one clone for exchange at a later date.

For the year 2004, participating countries have confirmed the working plans. The participating countries will continue the discussion and to exchange ideas through e-mails.

Thailand has offered training on in vivo screening technique for thrip resistance in 2004 to researchers from Malaysia and Indonesia.

Session 5: Other activities

1. Mutation Breeding Database (MBDB)

It was reported that Japan has revised the system of database and sent ten copies of MBDB to each participating country and it is also available on FNCA web site.

2. Mutant Stock Repository (MSR)

It was agreed that MSR is temporarily frozen because the distribution and deposition of mutants from participating countries are currently restricted for regulations. However, since stocking the mutants under the framework of the FNCA will be useful in the future, MSR could be resumed in the future.

3. Mutation Breeding Manual

The contents of the manual was presented by representative of the Philippines. This manual will be finalized and published later.

Session 6: The future plans proposed and agreed by the participants.

1. Next Workshop

Indonesia will host the next Workshop. 2005 will be in Malaysia.

2. New Sub Project: Disease Resistance in Banana

Start of the "Disease Resistance in Banana" was agreed with participation of Indonesia, Malaysia, the Philippines and Vietnam. These four countries will participate in the Project Formulation Meeting for “Disease Resistance in Banana” to be held after this Workshop.

Technical visit:

 

Participants visited IRRI and research highlights were introduced the representative of IRRI.

Open Lecture:

The Open Lecture with a theme on “Mutation Breeding of Ornamentals” was held on September 26 in the PNRI Auditorium. The public of around 60 people was also invited to attend this open lecture.



MINUTES of FNCA 2003 Workshop on Mutation Breeding
September 22-26, 2003
Cavite and Manila, the Philippines


   Following the agreement at the Fourth Coordinators Meeting in March, 2003 in Okinawa, Japan, the FNCA 2003 Workshop on Mutation Breeding took place as follows

Date: Monday, September 22 - Friday, September 26, 2003
Venue:
Manila Southwoods Manor, Carmona, Cavite, Philippines and
Philippine Nuclear Research Institute, Manila, Philippines
Host Organizations:
Department of Science and Technology (DOST)
Philippine Nuclear Research Institute (PNRI)
Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
In Cooperation with: Japan Atomic Industrial Forum Inc. (JAIF)
Working Language: English
Participants: Total 24 from 8 countries, i.e. China, Indonesia, Japan, Korea, Malaysia, the Philippines, Thailand and Vietnam

   At Session 1 “Presentation of Country Reports”, a total of seven papers on the current status of “Techniques on Reproductive Pattern in Root Crops” in the participating countries were presented to share their experiences.

   At Session 2 “Invited Paper”, Dr. J. Eusebio, PCARRD, presented a paper on Root Crops R & D Status and Directions: Philippines.

   At Session 3 “Drought Tolerance in Sorghum and Soybean Project”, Dr. Shigemitsu Tano, Project Leader of Japan presented the “Strategy for Drought Tolerance in Sorghum and Soybean Project and Insect Resistance in Orchid Project”. Country Reports concerning the “Drought Tolerance in Sorghum and Soybean” were presented by participating countries and “Action Plan for Drought Tolerance in Sorghum and Soybean” was discussed. The points agreed upon are as follows:

   China and Indonesia have been involved in mutation breeding of sorghum project, while the Philippines and Vietnam participated in the soybean project. Following the agreement during the project formulation meeting, breeding materials of sorghum and soybean have been exchanged between the respective participating countries.

  - The breeding materials have been included in mutation breeding program in each participating country, and results from early stage of research were reported. Each participating country found that it was beneficial to join the project, and would use the breeding materials to produce new improved varieties as the final goal

  - During the discussion, it was suggested that development method of increasing plant genetic variability by induction of mutation should be considered. With regard to this, the utilization of ion-beam for plant breeding purposes was encouraged. Since the ion-beam facility is available in China and at JAERI, Japan, The Office of Coordinator of Japan will facilitate a system of research collaboration by bridging the gap among participating countries.

   Malaysia and Indonesia expressed their interest to participate in the research project of drought tolerance in soybean, starting next year and was agreed by the group

   At Session 4, after presentation on “Insect Resistance in Orchid Project” by participating countries, “Action Plan for Insect Resistance in Orchid” was discussed. The points agreed upon are as follows:

-

Two clones from Thailand and one clone from Malaysia were exchanged at the Workshop. Indonesia will send one clone for exchange at a later date

-

For the year 2004 participating countries will implement the working plans agreed upon during the formulation meeting in Beijing which include

  1. Irradiation of protocorm like bodies (PLBs) of clones received from each participating country
  2. Determination of radiation sensitivity and optimum dose
  3. Development of in vitro selection techniques
  4. Multiplication of irradiated PLBs up to three subcultures

   In addition, the participating countries will continue discussion and to exchange ideas through e-mails in designing detailed technical procedures especially on in vitro selection techniques. Thailand has offered training on in vivo screening technique for thrip resistance in 2004 to researchers from Malaysia and Indonesia.

   At Session 5 on “Sub Projects”, three ongoing sub projects, i.e. “Mutation Breeding Database (MBDB)”, “Mutation Breeding Manual” and “Mutant Stock Repository (MSR)” were discussed.

(1) Mutation Breeding Database (MBDB)

   Dr. S. Tano reported that the Office of Coordinator of Japan has revised the system with ACCESS 2000 and sent ten copies of MBDB to each participating country in CD-ROM for their use. MBDB was also linked to FNCA website. However, participating countries indicated that both the MBDB CD-ROM and MBDB website were not accessible.

   The Office of Coordinator of Japan will include the introduction page in the MBDB website on the type of information available in the database. Help Manual on the use of the database will be made available for downloading.

The followings were suggested and agreed upon:

  - The problems in accessing the MBDB on the website will be rectified
- Request to access certain information in the MBDB in the website will be granted to individuals upon approval by the Office of Coordinator of Japan.
- The Office of Coordinator of Japan will seek permission from researchers listed in the database for confidential information to be accessed.

 

(2) Mutant Stock Repository (MSR)

   Dr. S. Tano introduced present situation of MSR use. MSR has been managed under cooperation of Thailand and the Philippines after Bangkok Workshop in 2001. However, because of regulations, the distribution and deposition of materials from participating countries are currently restricted. Since stocking the mutants under the framework of the FNCA will be useful in the future, MSR will be put on hold temporarily and can be resumed in the future.

(3) Mutation Breeding Manual

   Dr. Florencio-Isagani S. MEDINA, III, presented the contents of the manual. The manual consists of three parts, namely

PART I:

The Essential Basic Information.
This covers the necessary background a beginner needs to know about the principles and practices of mutation.

PART II:

Induced Mutation Techniques
This is a discussion of mutation breeding techniques and methodologies used by members for mutation induction.

PART III: Cell and Molecular Biotechnology Techniques in Mutation Breeding

   This part presents the different methodologies in cell and molecular biotechnology as it applies to mutation breeding either in the preparation of the samples prior to or after exposure to mutagenic treatments for mutation induction.

   Each participating country is requested to send comments on the manual, and unless there are no additional contributions, then the manual is considered complete and will be published in the Website. This will be finalized depending on the comments of Dr. Etsuo Amano, one of the editors. The Office of Coordinator of Japan will be requested to reproduce a limited number of copies and distribute to the Project Leaders in each country.

   On Wednesday, September 24, the participants went on a technical visit to the IRRI. The participants expressed their sincere gratitude to the organizers. Participants visited Riceworld Museum and Learning Center and IRRI's Rice Gene Bank. Participants were introduced highlights, results on plant molecular biology laboratory in the last time up to now, especially in resulting and directing on application new molecular technique in rice breeding with high yield, good quality and resistant to biotic and abiotic stress. IRRI achieved successful results of creation Bt rice and gold rice, and continue testing them in the glass house. All participants learned experiences and highlight results in rice genetics and breeding researches of IRRI. We appreciated effort of Workshop organaizers to be given chance of this technical visit to IRRI.

   At Session 6, future plans proposed and agreed by the participants were as follows,


1. Phase III

   Participants agreed to change the present way of information exchange (from theme to project/program meeting). In accordance with the consensus of previous Workshop, participants chose to focus on "Clonally Propagated Crops" for FNCA 2004 Workshop.

2. Next Workshop

   It was agreed that Indonesia would host the FNCA 2004 Mutation Breeding Workshop. All participants appreciated the candidature of Malaysia as the tentative venue for the 2005 Mutation Breeding Workshop.


3. “Disease Resistance in Banana”

   Start of the "Disease Resistance in Banana" was agreed with participation of four countries, i.e. Indonesia, Malaysia, the Philippines and Vietnam. These four countries will participate in the Project Formulation Meeting for “Disease Resistance in Banana” to be held on September 29 and 30 in Manila, the Philippines. It is expected that status of banana research, proposals from each country and working plan will be presented, discussed and formulated.

   The Minutes were discussed and agreed upon by all participants in the Workshop. This will be reported at the Fourth FNCA Coordinators Meeting to be held in March 2004 in Tokyo, Japan.

    Open Lecture with a theme on “Mutation Breeding of Ornamentals” was held on Friday, September 26 in the PNRI Auditorium. The lecture was found significant and very useful to disseminate information on Mutation Breeding technology to farmers and breeders. The public of around 60 people was also invited to attend this open lecture.



2003 FNCA Mutation Breeding Workshop
Southwoods Country Club, Cavite, Philippines
September 22-26, 2003



Date

:

Monday, September 22 - Friday, September 26, 2003

Venue

:

Manila Southwoods Manor Hotel, Manila, the Philippines

Host Organization

:

Department of Science and Technology (DOST)
Philippine Nuclear Research Institute (PNRI)
Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)

In Cooperation with

:

Japan Atomic Industrial Forum Inc. (JAIF)

Working Language

:

English

Mon, 22 September

:

 

08.20-08.50

:

R E G I S T R A T I O N

08.50-09.00

:

Welcome Address
Dr. Alumanda M. Dela Rosa
Director
Philippine Nuclear Research Institute (PNRI), Philippines

09.00-09.10

:

Remarks
Mr.Nobuo Tachikawa, MEXT

09.10-09.30

:

Remarks & Progress Report on FNCA Activities
“Review of FNCA Activities”
Dr. Sueo Machi, JAIF
FNCA Coordinator of Japan

09.30-09.45

:

Keynote Address
Dr. Estrella F. Alabastro
Secretary, Department of Science and Technology, Philippines

09.45-10.00

:

B R E A K

SESSION I Presentation of Country Reports
“Techniques on Reproductive Pattern in Root Crops”
Chair: Thailand

10.00-10.30

:

Country Report of China
“Root Crop Improvement by Mutation Techniques in China”
Prof. Liu Luxiang, CAAS

10.30-11.00

:

Country Report of Indonesia
“Selection of Sweetpotato (Ipomoea batatas) Clones Resistant to Sweetpotato weevil (Cylas formicarius F) through Induced Mutation”
Dr. Muhammad Jusuf Yakub
Research Institute For Legume And Tuber Crops

11.00-11.30

:

Country Report of Japan
“Recent Progress of Sweetpotato Breeding in Japan with Consumer Oriented Strategies”
Dr. Makoto Nakatani, NARO

11.30-12.00  

Country Report of Korea
“Techniques on Reproductive Pattern of Sweetpotato in Korea”
Mr. Mi-Nam Chung, RDA

12.00-13.30

:

L U N C H

Continuation of Session I
Chair: Indonesia

13.30-14.00

:

Country Report of Malaysia
“Status Report on Breeding of Root Crops in Malaysia”
Dr. Mohamad bin Osman, Universiti Kebangsaan Malaysia

14.00-14.30

:

Country Report of the Philippines
“Status of Mutation Breeding in Root Crops in the Philippines”
Ms. Avelina G. Lapade, PNRI

14.30-15.00

Country Report of Thailand
“Present Status of Cassava Breeding in Thailand”
Prof. Dr. Siranut Lamseejan

15.00-15.30

:

Country Report of Vietnam

15.30-15.45   B R E A K

SESSION 2: Invited Lectures
Chair: Korea

15.45-16.15

:

Invited Paper 2
Dr. Jocelyn E. Eusebio
Director of the Crops Research Division
PCARRD, Philippines

18.30

:

D I N N E R
Hosted by the Philippines and Japan

Tue, 23 September
Session 3:
Drought Tolerance in Sorghum and Soybean Project

Co-Chairs: Malaysia and Thailand

09.00-09.10

:

“Strategy for Drought Tolerance in Sorghum and Soybean Project and Insect Resistance in Orchid Project”
Dr. Shigemitsu Tano
Project Leader of Japan

09.10-09.30

:

Status Report on Sorghum of Indonesia
“FNCA’s Multilateral Research Program 1: Mutation Breeding In Sorghum for Drought Tolerance In Indonesia”
Dr. Soeranto Human, BATAN

09.30-09.50

:

Status Report on Sorghum of China
“Report on Current Status and Future Plan of Mutation Breeding in Sorghum for Drought Tolerance in China”
Prof. Liu Luxiang, CAAS

09.50-10.05

:

Special Lecture on Sorghum of Japan
“The Recent Condition of Sorghum Breeding in Japan”
Dr. Shigemitsu Kasuga, Shinsyu University

10.05-10.25

:

Status Report on Soybean of the Philippines
“Status Report on the FNCA Multilateral Research Program 1/ (Mrp-1) on Drought Tolerance of Soybean (Glycine Max L.)”
Mr. Apolinar B. Asencion, PNRI

10.25-10.45

:

Country Report on Soybean of Vietnam
“Development of Drought Tolerance Soybean in Vietnam”
Prof. Dr. Dong Huu Nguyen, MARD

10.45-11.00

:

B R E A K
Co-Chair: Japan and Vietnam

11.00-12.15

:

Action Plan for Drought Tolerance in Sorghum and Soybean Project

12.15-13.30

:

L U N C H

SESSION 4: Insect Resistance in Orchid Project
Co-Chairs: China and Vietnam

13.30-13.50

:

Status Report on Orchid of Indonesia

13.50-14.10

:

Status Report on Orchid of Malaysia
Dr. Mohd.Nazir Basiran, MINT

14.10-14:30

:

Status Report on Orchid of Thailand
“Insect Resistance Induction in Orchids of Thailand”
Ms. Chitrapan Piluek, KU

14:30-14:45

B R E A K

14.45-16.00

:

Action Plan for Insect Resistance in Orchid Project
SESSION 5: Sub Projects
Co-chairs: China and Indonesia

16.00-17.00

:

(1) Mutation Breeding Database (MBDB)
Dr. Shigemitsu Tano
Project Leader of Japan

 

:

(2) Mutant Stock Repository (MSR)
Dr. Shigemitsu Tano
Project Leader of Japan

 

:

(3) Mutation Breeding Manual
Dr. Florencio-Isagani Medina III
The Philippines

Wed, 24 September

Technical Visit: The International Rice Research Institute (IRRI)
Los Banos, Laguna

Thu, 25 September

SESSION 6: Round Table Discussion for Wrap-up
Co-Chairs: Japan and Philippines

09.00-11.00

:

General Discussion

11.00-11.30

:

Drafting Minutes

11.30-11.45

:

Wrap-up: Adoption of the Minutes

11.45-12.00

:

Closing Remarks
Dr. Shigemitsu Tano
Project Leader of Japan

12.00-13.30

:

L U N C H

13.30-14.00

:

Check out – Southwoods Manor Golf and Country Club
Move to Shangrila Plaza Hotel, Madaluyong City

14.00-16.00

:

Leave Southwoods Manor to Shangrila EDSA Plaza

Fri, 26 September

:

 

Open Lecture: Mutation Breeding of Ornamentals
           PNRI Auditorium

08:30-09.00

:

Registration

09:00-09.15

:

Welcome Address
Dr. Alumanda M. dela Rosa
Director, PNRI

09.15-09.45

:

Keynote Speaker

09.45-10.15  

Accomplishments, Prospects and Future Plans for Ornamental Horticulture
PCARRD, Philippines

10.15-10.45  

Dr. Nazir Basiran, MINT, Malaysia

10.45-11.00  

B R E A K

11.00-11.30  

“Enticing Thai Ornamental Growers in Sharing Our Mutation Breeding Technology”
Dr. Siranut Lamseejan
Kasetsarat University, Thailand

11.30-12.00  

Open Forum

13.30-14.00

:

Avelina G. Lapade, PNRI

14.00-14.15

:

Closing Remarks
Dr. Alumanda M. dela Rosa
Director, PNRI

14.15-16.00

:

Visit to PNRI Irradiation Facility, Greenhouse and Tissue Culture Laboratories of Agricultural Research
Section, ARD/PNRI


The List of Participants
of the 2003 FNCA Workshop on Mutation Breeding
September 22 – 26, 2003
Manila, the Philippines


Country

Name

Address

China

Prof. Wang Zhidong

Project Leader of Mutation Breeding Project of China
Deputy Director General
Institute for Application of Atomic Energy (IAAE)
Chinese Academy of Agricultural Sciences (CAAS)

Prof. Liu Luxiang

Director
Dept. of Plant Mutation Breeding & Genetics
Institute of Crop Science / IAAE, CAAS

Indonesia

Dr. Masrizal Project Leader of Mutation Breeding Project of Indonesia
Head of Division, Agriculture
CRDIRT - NNEA (BATAN)
Dr. Muhammad Jusuf Yakub Sweetpotato Breeder
Breeding
Research Institute For Legume And Tuber Crops
Dr. Soeranto Human Head of Plant Breeding Section
Agriculture
CRDIRT - NNEA (BATAN)

Japan

Dr. Shigemitsu Tano

Project Leader of Mutation Breeding Project of Japan
Ex-Professor
The University of Tokyo

Dr. Makoto Nakatani

Head
Sweetpotato Breeding Lab.
National Institute of Crop Science
National Agricultural Research Organization

Dr. Shigemitsu Kasuga

Associate professor
Agronomy Lab.
Education and Research Centre of ALPINE Field Science (AFC)
Faculty of Agriculture
Shinshu University

Dr. Minoru Nishimura

Head of Mutation Genetics Laboratory
Institute of Radiation Breeding
National Institute of Agrobiological Sciences (NIRS)

Dr. Sueo Machi

FNCA Coordinator of Japan,
Senior Managing Director
Japan Atomic Industrial Forum (JAIF)

Mr. Nobuo Tachikawa

Special Staff (in charge if International Cooperation)
Atomic Energy Division
Research and Development Bureau
Ministry of Education, Culture, Sports, Science and Technology (MEXT)

Ms. Mari Miura

Secretariat,
Asia Cooperation Center (ACC)
Japan Atomic Industrial Forum (JAIF)

Korea

Mr. Mi-Nam CHUNG

Researcher of Sweetpotato Research Lab.
Mokpo Experiment Station
National Honam Agricultural Experiment Station, RDA

Malaysia

Dr. Mohd.Nazir Basiran

Project Leader of Mutation Breeding Project of Malaysia,
Group Manager
Agrotechnology and Bioscience
Malaysian Institute for Nuclear Technology Research (MINT)

Dr. Mohamad bin Osman

Associate Professor
School of Environmental and Natural Resource Sciences, FST
Universiti Kebangsaan Malaysia

Philippines

Ms. Avelina Gutierrez Lapade

Project Leader of Mutation Breeding Project of the Philippines
Supervising Science Research Specialist & Head ARG
Agricultural Research Group, Atomic Research Division
Philippine Nuclear Research Institute (PNRI)

Mr. Apolinar Bergula Asencion

Scientist I
Agricultural Research Group/Atomic Research Division
Philippine Nuclear Research Institute (PNRI)

Dr. Florencio-Isagani S. Medina III

Graduate Professor & Career Scientist III (Retired)
C/o Cytogenetics Laboratory , Biomedical Research Group, Atomic Research Division
Philippine Nuclear Research Institute (PNRI) - Consultant

Ms. Ana Maria Serrano Veluz

Science Research Specialist II
Agricultural Research Group, Atomic Research Division
Philippine Nuclear Research Institute (PNRI)

Mr. Alfonso Obar Grafia

Senior Science Research Specialist
Agricultural Research Group, Atomic Research Division
Philippine Nuclear Research Institute (PNRI)

Thailand Prof. Dr. Siranut Lamseejan Project Leader of Mutation Breeding Project of Thailand,
Professor and Consultant
Gamma Irradiation Service and Nuclear Technology Research Center
Kasetsart University (KU)
Ms. Chitrapan Piluek Assistant Professor
Horticulture Department
Faculty of Agriculture
2001-2003 Kasetsart University

Vietnam

Prof. Dr. Quy Duy Trab

Director General
Agricultural Genetics Institute
Ministry of Agriculture and Rural Development

Prof. Dr. Dong Huu Nguyen

Director
Centre for Plant Biotechnology, Agricultural Genetics Institute


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