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FNCA 2008 Mutation Breeding Project Workshop
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Report of
FNCA 2008 Workshop on Mutation Breeding

October 27 - 31, 2008
Dalat City, Viet Nam

Following the agreement at the 9th Coordinators Meeting 2008 in Tokyo, Japan, the FNCA 2008 Workshop on Mutation Breeding took place as follows;

Date : October 27-31, 2008
Venue : Blue Moon Hotel, 4 Phan Boi Chau, DaLat City, Viet Nam
Host Organization : Centre for Applications of Nuclear Technique in Industry (CANTI), Vietnam Atomic Energy (VAEC)
Ministry of Education, Culture, Sport, Science and Technology (MEXT), Japan
Agricultural Genetics Institute (AGI), Vietnam Academy of Agricultural Sciences (VAAS)
Secretariat : Centre for Applications of Nuclear Technique (CANTI)
Nuclear Safety Research Association (NSRA)
Participants : Total 45 from 8 countries, Bangladesh, China, Indonesia, Japan, Malaysia, the Philippines, Thailand and Viet Nam

Opening Session and Open Lecture

This Workshop began with the opening remarks by Assoc. Prof. Dr. Le Huy Ham, Director General of Institute of Agricultural Genetics (AGI), and followed by Dr. Hitoshi Nakagawa delivered the opening address. And welcome address was delivered by Assoc Prof. Dr. Le Xuan Tham. Then Assoc. Prof. Dr. Nguyen Huu Quang, Director of Centre for Application of Nuclear Technology in Vietnam delivered the opening declaration.

And eleven special lectures were presented by Japan, Vietnam and China as follows.

Dr. Nguyen Huu Quang
Dr. Nguyen Huu Quang
Special Lecture 1 “Recent Progress of Radiation-Applied Biotechnology in Japan”
(Dr. Tanaka, JAEA, Japan)
Special Lecture 2 “Mutation Breeding and Biological Researches by the Use of Gamma Ray Irradiation in Japan”
(Dr. Hitoshi Nakagawa, Project Leader of Japan)
Special Lecture 3 “Crop Mutation Breeding in Vietnam: Current Status, Opportunities and the Way Forward” (Dr. Mai Quang Vinh, AGI, Vietnam)
Special Lecture 4 “Overview on Methodology, Strategy of Crops Quality Improvement Through Induced Mutations and Biotechnology”
(Dr. Bui Chi Buu, Vietnam)
Special Lecture 5 “Achievements, Experienced Lessons of Mutation Breeding in Improving Quality and Important traits of Rice in South Vietnam”
(Dr. Do Khac Thinh, Vietnam)
Special Lecture 6 “Improvement of Combined Aromatic Rice with High Quality by Using Mutation and Related Techniques” (Dr. Le Xuan Tham, Vice Director of Bureau of Science and Technology, Lamdong Province Vietnam)
Special Lecture 7 “Development of Rice (Oryza Sativa L.) with Low Phytic Acid in Vietnam” (Dr. Nguyen Thi Lang, Vietnam)
Special Lecture 8 “Review of Mutation Breeding of Rice in China”
(Mr. Luo Ju, CNRRI, China)
Special Lecture 9 “Research on Rice Plant Mutation Breeding”
(Dr. Minoru Nishimura, NIAS, Japan)
Special Lecture 10 “Use of in vitro flowering technique and γ - ray irradiation for the studyof in vitro mutation breeding”
(Dr. Nguyen Tien Thinh, Nuclear Research Institute)
Special Lecture 11 “Nuclear technology in stimulating somatic embryo development of Lilium spp.” (Dr. Duong Tan Nhut, Vietnam)

Sub-Project Meeting on Composition or Quality in Rice

Rice Varieties of Vietnam
Rice Varieties of Vietnam

The country reports were presented and discussed by participants as follows. At the round table discussion, 5-year working plan made in 2007 was amended and concluded as follows.

Conclusion of the session

  1. Standard variety, IR64 for indica rice and Koshihikari for japonica rice, should be added to the material for the evaluation of rice quality.
  2. Standardization of evaluation techniques for content and composition of 1) amylose, 2) protein, 3) phytic acid, and 4) aroma shall be determined.
  3. Evaluation methods of amylose and protein have been determined and mailed to all the participants.
  4. Evaluation method of phytic acid will be informed by Thailand.

Report of Sub-Project Meeting on Disease Resistance in Banana

The report on Sub-Project on Disease Resistance in Banana was presented by Dr. Rusli Ibrahim (Malaysian Nuclear Agency).

He reported that participating countries such as Philippines, Vietnam and Malaysia have successfully achieved the objectives of the project by identifying promising mutant lines which are disease resistant (BBTV, Fusarium and Nematode) and agreed to extend this project until 2010 as Phase II towards achieving the final goal of obtaining resistant new varieties of banana.

All participants agreed with the suggestion of extending this Sub-Project for another 2 years until 2010. This proposal will be discussed for approval at the Tenth Coordinators Meeting to be held in March 2009 in Tokyo, Japan.

Sub-Project Insect Resistance in Orchid

The report on Sub-Project on Insect Resistance in Orchid was presented by Dr. Rusli Ibrahim (Malaysian Nuclear Agency).

He reported that in vitro screening of D. mirbellianum showed an increase in resistance towards mite infestation at higher doses above 1.0Gy. In addition, in vivo screening test on flowering plants has so far generated 2 mutant lines with potential tolerance to thrips.

Technical Visit

The participants made Technical Visit to Nuclear Research Institute, Dalat Hasfarm, Langbiang Farm and Nha Ho Research Institute on Cotton and Rural Development.

Nuclear Research Institute Dalat Hasfarm Langbiang Farm
Nuclear Research Institute Dalat Hasfarm Langbiang Farm

Minutes

The Minutes were discussed and agreed upon by all participants in the workshop. This will be reported at the Tenth Coordinators Meeting to be held in March 2009 in Tokyo, Japan.

Variety of Rice Grape farm Nha Ho Research Institute on Cotton and Rural Development
Variety of Rice Grape farm Nha Ho Research Institute on Cotton and Rural Development

Minutes of
FN CA 2008 Workshop on Mutation Breeding

October 27 - 31, 2008
Dalat City, Viet Nam


Date : October 27-31, 2008
Venue : Blue Moon Hotel, 4 Phan Boi Chau, DaLat City, Viet Nam
Host Organization : Centre for Applications of Nuclear Technique in Industry (CANTI), Vietnam Atomic Energy (VAEC)
Ministry of Education, Culture, Sport, Science and Technology (MEXT), Japan
Agricultural Genetics Institute (AGI), Vietnam Academy of Agricultural Sciences (VAAS)
Secretariat : Centre for Applications of Nuclear Technique (CANTI)
Nuclear Safety Research Association (NSRA)
Participants : Total from 8 countries, Bangladesh, China, Indonesia, Japan, Malaysia, the Philippines, Thailand and Viet Nam (Attachment II)
Program : Attachment I

Opening Session

The session began with the opening remarks by Assoc. Prof. Dr. Le Huy Ham, Director General of Institute of Agricultural Genetics (AGI), and followed by Dr. Hitoshi Nakagawa delivered the opening address. And welcome address was delivered by Assoc Prof. Dr. Le Xuan Tham. Then Assoc. Prof. Dr. Nguyen Huu Quang, Director of Centre for Application of Nuclear Technology in Vietnam delivered the opening declaration.

Session 1  Open Lecture 1

Project Review of FNCA Mutation Breeding Project was delivered by Dr. Hitoshi Nakagawa, and followed by nine special lectures were presented by Japan, Vietnam and China as follows.

Special Lecture 1 “Recent Progress of Radiation-Applied Biotechnology in Japan” (Dr. Tanaka, JAEA, Japan)
Special Lecture 2 “Mutation Breeding and Biological Researches by the Use of Gamma Ray Irradiation in Japan” (Dr. Hitoshi Nakagawa, Project Leader of Japan)
Special Lecture 3 “Crop Mutation Breeding in Vietnam: Current Status, Opportunities and the Way Forward” (Dr. Mai Quang Vinh, AGI, Vietnam)
Special Lecture 4 “Overview on Methodology, Strategy of Crops Quality Improvement Through Induced Mutations and Biotechnology”
(Dr. Bui Chi Buu, Vietnam)
Special Lecture 5 “Achievements, Experienced Lessons of Mutation Breeding in Improving Quality and Important traits of Rice in South Vietnam”
(Dr. Do Khac Thinh, Vietnam)
Special Lecture 6 “Improvement of Combined Aromatic Rice with High Quality by Using Mutation and Related Techniques” (Dr. Le Xuan Tham, Vice Director of Bureau of Science and Technology, Lamdong Province Vietnam)
Special Lecture 7 “Development of Rice (Oryza Sativa L.) with Low Phytic Acid in Vietnam” (Dr. Nguyen Thi Lang, Vietnam)
Special Lecture 8 “Review of Mutation Breeding of Rice in China”
(Mr. Luo Ju, CNRRI, China)
Special Lecture 9 “Research on Rice Plant Mutation Breeding”
(Dr. Minoru Nishimura, NIAS, Japan)

Dr. Tanaka reported that not only mutation breeding but also new biotechnology and biology have been created by means of ‘Quantum Beam Technology’. New plant varieties were effectively induced by ion beams. Heavy-ion microbeam technology showed heavy ions could overcome tumor radioresistance. Positron emitting tracer imaging system showed imaging and quantitative analysis of nutrient and heavy-metal transportation in a rice plant.

Session 2  Sub- Project Meeting on Composition or Quality in Rice

A special lecture is delivered by Dr. Nguyen Duc Thanh of Vietnam, the title was “Induced mutagenesis and molecular technologies for development of drought resistance in quality rice” and the country reports were presented by participants. (Attachment III)

1. Bangladesh, Dr. Lokman Hakim

Four salt tolerant rice landraces were collected from the two coastal districts of Bangladesh and their protein content were estimated along with IR 64 following micrio-Kjeldahl method. The protein content ranged from 7.90 to 9.94% while it was 8.06 for IR 64. M1 plants of these salt tolerant rice were raised in the field from gamma irradiated (350 Gy) seeds in boro season (December, 07-May, 08). Mutated M1 plants of Morichshail (early maturing and blackish panicle) were harvested separately while M1 plants of each population were harvested separately in bulk and were grown separately as M2 populations in the following aman season (July-December, 08). In addition, seeds of two salt tolerant rice Ashfal and Moricshail were also irradiated this year (aman, 2008) with 350 Gy gamma ray and M1 plants were grown in the field. Optimization of media composition for in vitro regeneration of plants from mature seeds of salt tolerant rice has been established. Irradiated seeds of salt tolerant rice Takshoyl were also used for in vitro organogenesis and 16 green plants of the said variety are now growing in the pot with vigor.

2. China, Dr. Luo Ju

Rice mutation breeding of low amylose content (LAC) in China had been started since 2006. Nineteen collected restorers, as CR10, CR17, CR128, CR86, CR501, had been irradiated using gamma ray with doses of 250 for LAC breeding. Selections were conducted in M2 populations with desirable agronomic traits and 578 M2 lines were obtained in 2007. 435 M3 lines were primary analyzed for low amylose content with rapid measurement in 2008 and 12 mutant lines from 3 restorers were preserved. Among them, 3 lines crossed with the other varieties with good quality and 6 crosses were obtained.

3. Indonesia, Dr. Masrizal

KI 237 and KI 432, derived from a cross of Indica var. IR36 and Japonica var. Koshihikari, were irradiated by 200 Gy gamma rays. Selections were conducted in M2 populations with emphasize on plant height and days to heading. One dwarf M3 line, two semi-dwarf M3 lines, and one early maturity line were selected. Five hundreds of unselected M3 lines are also maintained for screening of amylose contents.

4. Japan, Dr. Minoru Nishimura

The mutant strains of Koshihikari obtained by gamma ray irradiation and EMS treatment were selected and backcrossed with Koshihikari to eliminate the undesirable characters except the low amylase trait. Amylose content of these F3 lines varied from 2.5% to 10.0%. Three rice varieties, namely Hitomebore, Akitakomachi and Nipponbare were irradiated with gamma rays and an ion beam. From these M2 seeds, we newly screened six mutants with low amylose contents.

5. Malaysia, Dr. Rusli Bin Ibrahim

This Project is focused on developing new rice cultivars with high yield under minimal water requirement. Seeds of selected elite genotypes of MR211 and MR219 were irradiated with 300 and 400Gy of gamma rays and exposed to selection pressure using different levels of PEG. A total of 38 potential mutants families from MR211 and MR219 were successfully evaluated in the field with minimal water requirement. These mutant lines will further tested for yield, resistance to blast and selection for low amylose content.

6. The Philippines, Dr. Alfonso O. Grafia

Seeds of rice variety IR72 previously exposed to 200 and 300 Co-60 gamma rays were planted in the field to isolate mutants with high protein with low to intermediate amylose contents. At harvest time the panicles were threshed and the seeds were prepared for protein and amylose analysis. Variations in protein contents were noted in the samples analyzed. Four mutants with low to intermediate amylose content were obtained among the samples analyzed. These mutants will be studied further to confirm whether they breed true or segregate in the M4 and later generations.

7. Thailand, Mr. Suniyom Taprab

Mutation breeding of LPA rice in Thailand had been started since 2003. I was started on a popular high yielding variety (SPR1) with 200 gray of gamma ray irradiation. Later, we induced mutation in another HYV such as RD23, PSL2, CNT1 and CNT80. In 2004, 619 M3-LPA mutant lines of SPR1 were derived and continuously selected for desirable agronomic traits in advance generation to M6. Three advanced homozygous lines of those M6 such as SPR1'03G1Cs-PTT-2-3-3-1-1, SPR1'03G1Cs-PTT-9-6-2-1-1 and SPR1'03G1Cs-PTT-1-8-2-1-1 are being evaluated for grain yield and quality in various environments. SPR1'03G1Cs-PTT-2-3-3-1-1 and SPR1'03G1Cs-PTT-9-6-2-1-1 had been analyzed for IP5, IP6 and Fe contents. The results showed lower quantity of IP5, IP6 and total IP in those two mutants than in original SPR1. Fe content in those two mutants and original SPR1 are almost equal. Allelism tests (lpa1-1) are being initiated. Currently, we detected 69 M5-LPA lines from RD23 and 67 M4-LPA lines from PSL2. In addition, M3 seed of CNT1 and CNT80 are being assayed for phytic acid content.

8. Vietnam, Dr. Le Xuam Tham

Under FNCA Program, in Vietnam during 2007-2008, some groups of breeders and biologists continue effective collaborations in efforts for pureline selections under field trials of rice mutants and their crossings with high quality and tolerance in Mekong River Delta (South Vietnam) and Red River Delta (North Vietnam).

  • Dr. Nguyen Thi Lang et al. in Omon Rice Research Institute (Cantho) have selected by MAS and obtained many promising lines for low phytic acid levels at M5-M6 from OM 1490 and OMCS 2000 varieties and screened some local cultivars.
  • Dr. Do Khac Thinh et al. in Institute of Agriculture Sciences, South Vietnam have continued field trials for some combinations of aromatic rice and mutant lines with wide adaptability.
  • Dr. Le Xuan Tham et al. in Provincial Department of Science & Technology Lamdong and Provincial Department of Agriculture & Rural Development Soctrang have released some promising pure lines and combined crossings of aromatic mutant lines in Soctrang Province (by center of Mekong Delta). These combinations of true breeds with special high quality were awarded National Medals of Labour in 2007-2008 and released for large scale productions in multilocations. Direct analysis of nutritive ingredients of aromatic rice combinations (2AP, Fe, P, Zn, Cr,... and protein, amylose), particularly some Red ST combined lines were shown to offer possible high Fe content germplasms for new varieties and further breeding.
  • Dr. Le Huy Ham et al. in Institute of Agriculture Genetics, Hanoi and other groups in North Vietnam have developed some useful markers strictedly related to genes and characters for selections of promising mutant lines with high yields and good tolerance to drought and salinity.

Conclusion of the session

  1. Standard variety, IR64 for indica rice and Koshihikari for japonica rice, should be added to the material for the evaluation of rice quality.
  2. Standardization of evaluation techniques for content and composition of 1) amylose, 2) protein, 3) phytic acid, and 4) aroma shall be determined.
  3. Evaluation methods of amylose and protein have been determined and mailed to all the participants.
  4. Evaluation method of phytic acid will be informed by Thailand.

The Workshop for Ion Beam in Japan

Dr. Tanaka presented the characteristics of ion-beam induced mutation in plants and how to prepare the material for the ion beam workshop. Ms. Koike, the secretariat office of FNCA in Japan, introduced a tentative schedule of the workshop in JAEA, Japan in January 2009.

Session 3  Special Lecture 2

The other two special lectures were delivered by Vietnam as follows.

Special Lecture 10 “Use of in vitro flowering technique and γ - ray irradiation for the studyof in vitro mutation breeding”
(Dr. Nguyen Tien Thinh, Nuclear Research Institute)
Special Lecture 11 “Nuclear technology in stimulating somatic embryo development of Lilium spp.” (Dr. Duong Tan Nhut, Vietnam)

Session 4  Report of Sub-Project Meeting on Disease Resistance in Banana

The report on Sub-Project on Disease Resistance in Banana was presented by Dr. Rusli Ibrahim (Malaysian Nuclear Agency) as Attachment IV.

Screening for Fusarium wilt disease resistance, were initiated by irradiating meristem cultures derived from suckers of popular cultivar Berangan (Musa spp. AAA) with a series of gamma ray doses from 0-100Gy. LD50 and LD100 obtained were 50Gy and 80Gy respectively. Four screening techniques against Fusarium wilt disease using artificial inoculation had been developed. At present, three potential mutants tolerance/resistance to Fusarium wilt were selected with improved characters such as early flowering, dwarf and high yield. These selected mutants will be further tested in multi-location trials and identification of specific molecular markers.

All participating countries such as Philippines, Vietnam and Malaysia have successfully achieved the objectives of the project by identifying promising mutant lines which are disease resistant (BBTV, Fusarium and Nematode). Achievement from this project will be published as proceedings and protocols for irradiation and screening for Fusarium, BBTV and Nematode. As a new member of this sub-project of banana, Bangladesh can adopt all the disease screening methods developed by other participating countries. All participating countries agreed to extend this project until 2010 as Phase II towards achieving the final goal of obtaining resistant new varieties of banana.

Session 5  Sub-Project Insect Resistance in Orchid

The report on Sub-Project on Insect Resistance in Orchid was presented by Dr. Rusli Ibrahim (Malaysian Nuclear Agency) as Attachment V.

This multilateral research program was initiated to induce Orchid mutants resistant to insect, particularly to mites and thrips. Protocorm-like bodies (PLB) of two variety of orchids, D. mirbellianum and D. Jayakarta were irradiated with ion beam at JAEA using doses ranging from 0-2Gy. In vitro screening of D. mirbellianum showed an increase in resistance towards mite infestation at higher doses above 1.0Gy. In addition, in vivo screening test on flowering plants has so far generated 2 mutant lines with potential tolerance to thrips (Attachment V).

Session 6  Round Table Discussion

Rice Sub-Project

All participants amended their 5-year working plans if any following their achievement of 2008 (Attachment VI)

Banana Sub-Project

All participants agreed with the suggestion of extending this Sub-Project for another 2 years until 2010. This proposal will be discussed for approval at the Tenth Coordinators Meeting to be held in March 2009 in Tokyo, Japan.

Technical Visit

The participants made Technical Visit to Nuclear Research Institute, Dalat Hasfarm, Langbiang Farm and Nha Ho Research Institute on Cotton and Rural Development.

Dalat Hasfarm is biggest company in Dalat and their main products are carnation, chrysanthemum, rose, lily etc. They benefit from the mild climate of the Central Highlands in Vietnam. With 3 farms at different altitudes ranging from 1000 to 1500 meters above sea level, they are able to grow year round a wide assortment of cut-flowers, cut-foliage and young plants. It is established in 1994 and at the present total greenhouse area amounts to 40 hectares. 72% of flowers are exported to Japan, Australia, Singapore, Indonesia, Taiwan and Cambodia. Langbiang Farm has 7 ha of greenhouse and their main products are Lilium, cymbidium, lisianthus etc.

Introduction of a new mutation breeding program in Vietnam

Outline of the project plan on establishment of Gamma Field was introduced by Mr. Nguyen Huu Quang, Director of CANTI and bilateral cooperation to the member countries of FNCA particularly to Japan and Malaysia for the establishment, operation, maintenance and exploitation of Gamma Field were requested. After his presentation comments and suggestions were made from the member countries.

Minutes

The Minutes were discussed and agreed upon by all participants in the workshop. This will be reported at the Tenth Coordinators Meeting to be held in March 2009 in Tokyo, Japan.

Attachments:

  1. Program
  2. Participants List
  3. Sub-Project on Rice
  4. Sub-Project on Banana
  5. Sub-Project on Orchid
  6. Rice Sub-Project 5-year working plans 2008ver.

Program of
FNCA 2008 Workshop on Mutation Breeding

October 27 - 31, 2008
Dalat City, Viet Nam


Date : October 27 - 31, 2008
Venue : Blue Moon Hotel, 4 Phan Boi Chau, DaLat City, Viet Nam
Host Organization : Centre for Applications of Nuclear Technique in Industry (CANTI), Vietnam Atomic Energy (VAEC)
Ministry of Education, Culture, Sport, Science and Technology (MEXT), Japan
Agricultural Genetics Institute (AGI), Vietnam Academy of Agricultural Sciences (VAAS)
Secretariat : Nuclear Safety Research Association (NSRA)
Centre for Applications of Nuclear Technique (CANTI)
Working Language : English

Mon., 27th October
Opening Session
Chair : Vietnam
08:30 - 09:00 Registration
09:00 - 09:30 Opening session (CANTI - Organizers)
Welcome address (Representative of Lamdong province)
Opening address (AGI)
09:30 - 10:00 Group photo and Break
Session 1  Open Lecture
Chair : Vietnam
10:00 - 10:30 Project Review of FNCA Mutation Breeding Project
(Dr. Hitoshi Nakagawa, Project Leader of Japan)
10:30 - 11:00 Special Lecture 1: “Recent Progress of Radiation-Applied Biotechnology in Japan” (Dr. Tanaka, JAEA, Japan)
11:00 - 11:30 Special Lecture 2: “Mutation breeding and biological researches by the use of gamma ray irradiation in Japan”
(Dr. Hitoshi Nakagawa, Project Leader of Japan)
11:30 - 12:00 Special Lecture 3 : “Crop Mutation Breeding in Vietnam: Current Status, Opportunities and the Way Forward”
(Dr. Mai Quang Vinh, AGI, Vietnam)
12:00 - 13:30 Lunch
Cont. of Session 1  Open Lecture
Chair : Japan
13:30 - 14:00 Special Lecture 4: “Overview on Methodology, Strategy of Crops Quality Improvement Through Induced Mutations and Biotechnology”
(Dr. Bui Chi Buu, Vietnam)
14:00 - 14:30 Special Lecture 5: “Mutation Breeding and socio-economic impacts of mutant Rice in South Vietnam” (Dr. Do Khac Thinh, Vietnam)
14:30 - 15:00 Special Lecture 6 : “Improvement of Combined Aromatic Rice with High Quality by Using Mutation and Related Techniques”
(Dr. Le Xuan Tham, Vietnam)
15:00 - 16:00 Break and Poster
- Exhibition Viewing of Vietnam achievements in mutation breeding
- Discussion (Round Table)
16:00 - 16:30 Special Lecture 7: “Development of Rice (Oryza Sativa L.) with Low Phytic Acid in Vietnam” (Dr. Nguyen Thi Lang, Vietnam)
16:30 - 17:00 Special Lecture 8: “Review of Mutation Breeding of Rice in China”
(Mr. Luo Ju, CNRRI, China)
17:00 - 17:30 Special Lecture 9 about Rice (Dr. Minoru Nishimura, NIAS, Japan)
Tue., 28th October
Session 2  Sub-Project Meeting on Composition or Quality in Rice
Chair : China
09:00 - 09:30 Induced mutagenesis and molecular technologies for development of drought resistance in quality rice (Dr. Nguyen Duc Thanh, Vietnam)
09:30 - 10:00 Bangladesh
10:00 - 10:30 China
10:30 - 11:00 Break
11:00 - 11:30 Indonesia
11:30 - 12:00 Malaysia
12:00 - 13:30 Lunch
Session 2  Sub-Project Meeting on Composition or Quality in Rice
Chair : China
13:30 - 14:00 Japan
14:00 - 14:30 The Philippine
14:30 - 15:00 Thailand
15:00 - 15:15 Break
15:15 - 15:45 Vietnam (Dr. Le Huy Ham, Director of AGI)
15:45 - 16:30 Discussion about Rice Project
16:30 - 17:30 About Irradiation of Rice by TIARA in Japan (explanation)
(Dr. Atsushi Tanaka, Japan)
Wed., 29th October
Session 3  Special Lecture
Chair : Vietnam
09:00 - 09:20 Use of in vitro flowering technique and γ - ray irradiation for the study of in vitro mutation breeding,
Dr. Nguyen Tien Thinh - Nuclear Research Institute (Vietnam)
09:20 - 09:45 “Nuclear technology in stimulating somatic embryo development of Lilium spp.”,
Dr. Duong Tan Nhut, Vietnam
Session 4  Report of Sub-Project Meeting on Disease Resistance in Banana
Chair : Bangladesh
09:45 - 10:15 Presentation from Dr. Rusli Bin Ibrahim (Malaysia)
10:15 - 10:45 Discussion and Valuation of Sub-Project Disease Resistance in Banana
10:45 - 11:00 Break
Session 5 Sub-Project Insect Resistance in Orchid
Chair : Indonesia
11:00 - 11:30 Presentation from Dr. Rusli Bin Ibrahim (Malaysia)
11:30 - 12:00 Discussion on Insect Resistance in Orchid
12:00 - 13:30 Lunch
Technical Visit
13:30 - 17:00 Technical Visit in Dalat City
- Nuclear Research Institute
- Dalat Hasfarm (chysanthemum, rose, lily,carnation)
- Langbiang Farm (Cymbidiums, Eustomas, Phalaenopsis)
Thurs., 30th October
Session 6 Round Table Discussion
Chair : Thailand
08:45 - 10:15 Wrap-up the Minutes
10:15 - 10:30 Break
10:30 - 11:45 Adaption of Minutes
11:45 - 12:00 Closing
12:00 - 13:00 Lunch
Transportation from Dalat to Nha Trang
(Visit to Rice Farm)
Fri., 31st October
Technical Visit
07:30 - 16:00 Nha Ho (Dragon fruits, Cotton, Rice, Grape)

List of Participants
FNCA 2008 Workshop on Mutation Breeding

October 27 - 31, 2008
Dalat City, Viet Nam


No.

Country

Name / Position & Organization

1 Bangladesh
(PL)
Dr. Md. Lokman Hakim
Principal Scientific Officer
Institute of Food & Radiation Biology, Atomic Energy Research Establishment
Bangladesh Atomic Energy Commission (BAEC)
2 China Dr. Luo Ju
Associated Researcher
China national rice research institute (CNRRI)
3 Indonesia
(PL)
Dr. Masrizal
Assistant Deputy for Development of National Science and Technology System,
State Ministry for Research and Technology
4 Japan
(PL)
Dr. Hitoshi Nakagawa
Director, Institute of Radiation Breeding
Division of Genome and Biodiversity Research
National Institute of Agrobiological Sciences (NIAS)
5 Japan Dr. Atsusi Tanaka
Unit Manager
Radiation-applied Biology Division
Quantum Beam Science Directorate
Japan Atomic Energy Agency (JAEA)
6 Japan Dr. Minoru Nishimura
Senior Officer
Institute of Radiation Breeding
Division of Genome and Biodiversity Research
National Institute of Agrobiological Sciences (NIAS)
7 Japan
(Secretariat)
Ms. Aki Koike
International Affairs and Research Department
Nuclear Safety Research Association (NSRA)
8 Malaysia
(PL)
Dr. Rusli Bin Ibrahim
Senior Research Officer, Industrial Crop Group
Agrotechnology and Biosciences Division
Malaysian Nuclear Agency (Nuclear Malaysia)
9 The Philippines Dr. Alfonso O. Grafia
Senior Science Research Specialist
Philippine Nuclear Research Institute(PNRI)
10 Thailand
(PL)
Mr. Suniyom Taprab
Plant Breeder
Rice Department
Ministry of Agriculture and Cooperatives
11 Thailand
(Observer)
Miss Sunanta Wongpiyachon
Rice Department
Ministry of Agriculture and Cooperatives
12 Vietnam
(PL)
Dr. Mai Quang Vinh
Agriculture Genetic Institute - AGI
Vietnam Academy of Agricultural Sciences

Local Pariticpants

No.

Name

Position / Organization

1 Dr. Le Huy Ham Director,
Agriculture Genetic Institute - AGI
Vietnam Academy of Agricultural Sciences
2 Dr. Dang Trong Luong Head, Genetic Engineering Department
Agriculture Genetic Institute - AGI
Vietnam Academy of Agricultural Sciences
3 Ms. Dao Thi Thanh Bang Genetic Engineering Department
Agriculture Genetic Institute - AGI
Vietnam Academy of Agricultural Sciences
4 Mr. Khuat Huu Trung Genetic Engineering Department
Agriculture Genetic Institute - AGI
Vietnam Academy of Agricultural Sciences
5 Dr. Le Xuan Tham
(Invited speaker)
Vice Director
Bureau of Science & Technology, Lamdong Province, Vietnam
35 Tran Hung Dao, Dalat, Vietnam
6 Dr. Duong Tan Nhut
(Invited speaker)
Vice Director, Tay Nguyen Institute of Biology
116 Xo Viet Nghe Tinh, Da Lat
7 Prof. Dr. Bui Chi Buu
(Invited speaker)
Director General,
Institute of Agriculture Science of South Vietnam (IAS)
121 Nguyen Binh Khiem St., District 1, HCM City, VIETNAM
8 Dr. Do Khac Thinh
(Invited speaker)
Institute of Agriculture Science of South Vietnam (IAS)
121 Nguyen Binh Khiem St., District 1, HCM City, VIETNAM
9 Ms. Trinh Thi Luy
(Invited speaker)
Genetics and Plant Breeding, Cuu Long Delta Rice Research Institute, (CLRRI)
10 Dr. Nguyen Duc Thanh
(Invited speaker)
Institue for Biotechnology (IBT), Vietnamese Academy of Science and Technology
18 Hoang Quoc Viet, Hanoi, Vietnam
11 Dr. Vo Thuong Lan Department of Biology, University of Science, National University of Ha Noi, Viet nam
12 Dr. Nguyen Tien Thinh
(Invited speaker)
Nuclear research Institute,
Vietnam Atomic Energy Commission
01 Nguyen Tu Luc St., Da lat, Vietnam
13 Mr. Nguyen Van Binh Tay Nguyen Institute of Biology
116 Xo Viet Nghe Tinh, Dalat, Vietnam
14 Mr. Vu Quoc Luan Tay Nguyen Institute of Biology
116 Xo Viet Nghe Tinh, Dalat, Vietnam
15 Dr. Lam Ngoc Phuong Agriculture and Biological Application College, Cantho University,
30/4 Street, Cantho City, VietNam
16 Dr. Le Van Hoa Vice Dean, Agriculture and Biological Application College, Cantho University,
30/4 Street, Cantho City, VietNam
17 Dr. Le Viet Dung Vice Rector, Cantho University,
30/4 Street, Cantho City, VietNam
18 Dr. Nguyen Van Ket Dean, Agricultural and Forestry Faculty
Dalat University
01 Phu Dong Thien Vuong, Dalat, Vietnam
19 Mr. Mai Xuan Phan Faculty of Biotechnology,
Open University, HCMC,
97 Vo Van Tan, District 3, HCMC
20 Dr. Nguyen Nhu Toan Agro-Biology Faculty,
Ha Noi Pedagogical No. 2.
Xuan Hoa, Phuc yen, Vinh Phuc, Vietnam
21 Mr. Ho Quang Cua Soc Trang Agricultural and Rural Development Department
22 Mr. Tran Tan Phuong Soc Trang Agricultural and Rural Development Department
23 Ms. Nguyen Thi Thu Hien Agricultural Extension Centre
Soc Trang Agricultural and Rural Development Department
24 Mr. Vuong Ho Acting Director,
Bureau of Science & Technology, Soc Trang Province, 179 Tran Binh Trong, Soc Trang
25 Dr. Le Quang Luan Head, Biotechnology Laboratory, Centre for Nuclear Technique, Vietnam Atomic Energy Commission
217 Nguyen Trai, District 1, HCMC
26 Dr. Nguyen Mong Sinh Chairman, Union of Science and Technique Association of Lam dong Province
27 Mr. Nguy Xung Hung General Serectary, Union of Science and Technique Association of Lam dong Province
28 Mr. Nguyen Van Tu Director,
Agricultural Extension Centre,
Lamdong Science and Technology Department
29 Dr. Pham Huu Nhuong Deputy Director, Managerment board of High Technology Agricultural Zone, HCMC
30 Ms. Nguyen Tuong Mien Centre for Applications of Nuclear Technique in Industry - CANTI
13 Dinh Tien Hoang, Da Lat
31 Ms. Luong Thai Thanh Hang Centre for Applications of Nuclear Technique in Industry - CANTI
13 Dinh Tien Hoang, Da Lat
32 Ms. Le Thi Thanh Tam Centre for Applications of Nuclear Technique in Industry - CANTI
13 Dinh Tien Hoang, Da Lat
33 Mr. Le Tien Thanh Centre for Applications of Nuclear Technique in Industry - CANTI
13 Dinh Tien Hoang, Da Lat

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