Mutation Breeding workshop

- Project Review
- Introduction of the Project Leaders
- Mutation Breeding Publication Database
- Mutation Breeding Manual
- Sorghum & Soybean
- Insect Resistance in Orchid
- Disease Resistance in Banana
- Composition or Quality in Rice
- Rice for Sustainable Agriculture
- Papers for Project Outcome


FNCA 2005 Mutation Breeding

Sub-Project Meeting on Insect Resistance in Orchid
September 5 - 8, 2005
Bangkok, Thailand

FNCA 2005 Mutation Breeding Sub-Project Meeting on Insect Resistance in Orchid took place as follows

Date: September 5 - 8, 2005
Venue: Kasetsart University, Bangkok, Thailand
Host Organizations:
Kasetsart University (KU), Thailand
Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan
Secretariat: Japan Atomic Industrial Forum Inc. (JAIF)
1 each from Indonesia and Malaysia, 2 from Japan, and 4 from Thailand, total 8

[Opening Ceremony]
Welcome address was delivered by Deputy Director, Kasetsart University Research and Development Institute (KURDI).

[Special Lectures]
Dr. Shigeki Nagatomi presented a lecture on ‘Chronic and acute gamma irradiation on in vitro mutagenesis in plants’ and Dr. Valuli Rojanawong presented a lecture on ‘Thrips palmi melon thrips, palm thrips or cotton thrips insect pest of orchids in Thailand’.

[Progress Report on Insect Resistance in Orchid by Each Country]
Progress Reports on Insect Resistance in Orchid were presented by Indonesia, Malaysia and Thailand.


[Discussion on Present Status and Future Work Plan of the Project]
The progress reports presented by each country were discussed and the followings were agreed:

  1. the project will focus on selection of flower colour mutations and followed by selection for insect tolerance. Colour mutations are more important indicator for the variations derived from mutation induction and also for the commercial value of orchids.
  2. new work plan was formulated for 2006 and 2007 as in Attachment 3 to include screening for insect tolerance, flower colour mutants and meristem cloning of selected mutants.
  3. data of radiosensitivity of Dendrobium orchids should be obtained to determine the optimum dose of radiation.
  4. Indonesia will screen for thrips tolerance instead of mites.

[General Discussion on Methodologies, Problem and Solution]
In general, radiosensitivity to gamma rays was not clear on in vitro materials such as callus, PLBs etc. Hence additional investigation of radiosensitivity should be conducted using in vivo plant material, PLB and in vitro seedlings in wide range of radiation doses 0, 40, 80, 120, 160, 320, 640, 1280 Gy.
Split dose and chronic irradiation are preferable for mutation induction to reduce radiation damage.

[Midterm Report]
Midterm reports have been summarized as in Attachment 4.

[Technical Visit]
Participants made a visit to Jiad Orchid Nursery at Banglane, Nakorn Pathom Province and Thai Orchid Company’s nursery at Ratchaburi Province which have research collaboration with Kasetsart University in developing orchid variety through induced mutation. The participants were demonstrated to a good orchid nursery management and agricultural practices. In addition, the participants had a first hand knowledge about the disease problems and pests at the nursery level. The participants also observed the growth and morphological changes such as leaf color and shape, plant height and growth habit in irradiated orchid grown in both nurseries.

These field trip was followed by a visit to the export section of Thai Orchid Company Ltd in Bangkok to observe fumigation and packaging processes of orchid cut flowers in preparation for the export to world market. Thai Orchid Company Ltd is one of the top ten orchid exporters of Thailand. Participants had intensively discussed and exchanged ideas and knowledge about the orchid industry, the needs and the research interests of the industry with the President and the staffs of Thai Orchid Company Ltd. The company is very much interested in using ionizing radiation for orchid breeding.

The Minutes were discussed and agreed upon by all participants in the Meeting. This will be reported at the FNCA 2005 Mutation Breeding Workshop to be held in December 2005 in Malaysia.


  1. Program of the FNCA 2005 Mutation Breeding Sub-Project Meeting on Insect Resistance in Orchid
  2. Participants List of the FNCA 2005 Mutation Breeding Sub-Project Meeting on Insect Resistance in Orchid
  3. Working Plan of the FNCA Mutation Breeding Sub-Project on Insect Resistance in Orchid
  4. Midterm Report for FNCA Mutation Breeding Sub-Project on Insect Resistance in Orchid

Program of FNCA 2005 Mutation Breeding Sub-Project Meeting
on Insect Resistance in Orchid



5-8 September 2005



Gamma Irradiation Service and Nuclear Technology Research Center (GISC), Kasetsart University, Bangkok, Thailand



Sun, 4 September


Arrival of participants and stay at Rama Gardens Hotel

Mon, 5 September : Chairperson: Thailand



Welcome address - Deputy Director, KURDI



Special lecture - Dr. S. Nagatomi



Special lecture - Dr. Valuli Rojanawong



Progress report on Insect Resistance in orchid
- Indonesia
- Malaysia




    Chairpersons: Indonesia and Japan



Progress report on insect resistance in orchid
- Thailand






Discussion on present status and future work plan
of the project



Reception at Dairy Queen Restaurant
Stay at Rama Gardens Hotel

Tue, 6 September


Chairpersons: Malaysia and Japan



General discussion on methodologies , problem and solution
- Irradiation methodology and handling of irradiated materials
- Screening technique applicable for selection of insect resistance clones in irradiated materials In vivo and in vitro
- Isolation and multiplication of selected mutant clones
- Testing and evaluation of selected mutants at field level






Technical visit
Observe growth and morphological changes in irradiated orchids at Jiad Orchid Nursery, Nakorn Pathom Province
Stay at Rose Gardens Hotel

Wed, 7 September


Technical visit



Visit Floating Market at Damnernsaduak

10.00-13.30 :

Visit Thai Orchid Company's nursery at Ratchaburi Province
to observe insect control in commercial cut flower orchids
and investigate growth and flower changes in irradiated dendrobium orchids.



- Lunch



- Visit cut flower orchid exporter and observe fumigation and packaging of orchids for export at Thai Orchid Company, Bangkok
Stay at Rama Gardens Hotel

Thu, 8 September
: Chairpersons: Thailand and Japan
09.00-12.00 : Wrap up the minutes
12.00-13.00 : Lunch
13.00- :

Adoption of minutes and closing ceremony
Stay at Rama Gardens Hotel

Fri, 9 September : Departure of participants




1. Dr.Shigeki Nagatomi

Research Leader, Division of technology Development,Bio-oriented Technology Research Advancement Institulion(BRAIN)
National Agriculture and Bio-oriented Research Organization(NARO)

2. Ms.Yuko Wada

Asia Cooperation Center,Japan Atomic Industrial Forum,Inc(JAIF)

3. Dr.Mohd Nazir Basiran

Manager, Ornamental Group
Agrotechnology and Biosciences Division, Malaysian Institute for Nuclear Technology(MINT)

4. Ms.Ismiyati Sutarto

Plant Breeder, Horticulture/Agriculture, CRDIRT-BATAN
Jarkata, Indonesia

5. Dr.Siranut Lamseejan

Project Leader of Thailand
Professor and Consultant, Gamma Irradiation Service and Nuclear Technology Research Center(GISC),KU

6. Ms.Chitrapan Piluek

Assistant Professor
Horticulture Department,Faculty of Agriculture,KU

7. Dr.Valuli Rojanavongse


8. Ms.Pannee Pakkong

Assistant Professor and Head,
Gamma Irradiation Service and Nuclear Technology Research

Forum for Nuclear Cooperation in Asia