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Workshop

FNCA 2007 Mutation Breeding Project Workshop
Report>>   Minutes>>   Program>>   List of Participants>> Attachment VII>>

Report of
FNCA 2007 Mutation Breeding Project Workshop

November 19 - 23, 2007
Daejeon, Korea

Following the agreement at the Eighth Coordinators Meeting 2007 in Tokyo, Japan, the FNCA 2007 Workshop on Mutation Breeding took place as follows;

Date : November 19-23, 2007
Venue : Nuclear Human Resource Development Center (NHRDC)
Korea Atomic Energy Research Institute (KAERI)
Daejeon Metropolitan City
Korea
Host Organization : Ministry of Education, Culture, Sport, Science and Technology (MEXT), Japan
Korea Atomic Energy Research Institute (KAERI)
Secretariat : Nuclear Safety Research Association (NSRA), Japan
Participants : Total 21 from 8 countries, i.e. Bangladesh, Indonesia, Japan, Korea, Malaysia, the Philippines, Thailand and Viet Nam(Attachment II)

The participants from 8 countries
The participants from 8 countries

Opening Session and Open Lecture
This Workshop began with the opening address by Dr. Sung Kee Cho (Director, Radiation Research Center for Bio-technology, ARTI-KAERI), following the opening address, Project Review of FNCA Mutation Breeding Project was delivered by Dr. Hitoshi Nakagawa (Project Leader of Japan), and followed by three special lectures by Japan and Korea as follows.

- "Establishment of Ion Beam Technology"
  (Dr. Atsushi Tanaka, JAEA, Japan)
- "Mutation Breeding and Gene Isolation in Chrysanthemum"
  (Dr. Geung Joo Lee, KAERI, Korea)
- "High Amino Acid Accumulating Rice Mutants"
  (Dr. Dong Sub Kim, KAERI, Korea)

Country Report
Each participating country (Bangladesh, Indonesia, Japan, Malaysia, The Philippines, Thailand, Vietnam and Korea) presented their progress and general reports on Mutation Breeding in each country.

Sub-Project on Disease Resistance in Banana
The reports on Sub-project on Disease Resistance in Banana was presented by Malaysia and Indonesia, after that all participants discussed and decided about some themes about this sub-project as following.

- Indonesia has been terminated this sub-Project at the end of 2006.
- A Special meeting of sub-project on disease resistance in banana in 2008 is proposed to be held either in Malaysia, Philippines or Japan and all the participating countries submit the final report.

Sub- Project on Insect Resistance in Orchid
The final report of Sub-Project Meeting on Insect Resistance in Orchid was presented by Assoc. Prof. Arunee Wongpiyasatid (Kasetsart University, Thailand), and proposed to extend the project for another 2 years until 2009.
It is discussed among the participants and all participants agreed to extend and support the project for another 2 years until 2009.

Sub- Project Composition or Quality in Rice
At the Starting this session, Special lectures were delivered by Korea as follows.

- "Rice genetic resources by mutation and their exploitation"
  (Dr. Young Sub Shin, RDA, Korea)
- "Analysis methods for functional compounds in rice"
  (Prof. Young Sang Lee, Soonchunhyang Univ. Korea)

The reports on Sub-project on Composition or Quality in Rice was presented by all participating countries. After the presentations, the Agreement about exchange breeding material, a standard variety/ methodology, and general rules and regulations was made to proceed with the project the future work plan were discussed and accepted.

Round Table Discussion
The participants discussed about duties and responsibilities of the Project Leaders, and identified the role as follows.

- To collect all the progress reports from the Sub-Project Leaders
- To compile all the reports and send to the secretariat before the meeting
- To present the report during meeting if the Sub-Project Leader do not participate.
- The Project Leader, as a leader of mutation breeding researches in his or her country, will take part in coordinating and management of the Mutation Breeding Project and need to communicate with the Sub-Project Leader.

Technical Visit
Participants made a technical visit to Advanced Radiation Technology Institute (ARTI) of KAERI. They watched Video of introduction and explanation about ARTI, then visit at many laboratories and facility.


Minutes of
FNCA 2007 Mutation Breeding Project Workshop

November 19 - 23, 2007
Daejeon, Korea

The FNCA 2007 Mutation Breeding Project Workshop took place as follows;

Date : November 19-23, 2007
Venue : Nuclear Human Resource Development Center (NHRDC)
Korea Atomic Energy Research Institute (KAERI)
Daejeon Metropolitan City
Korea
Host Organization : Ministry of Education, Culture, Sport, Science and Technology (MEXT), Japan
Korea Atomic Energy Research Institute (KAERI)
Secretariat : Nuclear Safety Research Association (NSRA), Japan
Participants : Total 21 from 8 countries, i.e. Bangladesh, Indonesia, Japan, Korea, Malaysia, the Philippines, Thailand and Viet Nam
(Attachment II)

Opening Session

The session began with the opening address by Dr. Sung Kee Cho (Director, Radiation Research Center for Bio-technology, ARTI-KAERI).

Session 1   Open Lecture 1

Project Review of FNCA Mutation Breeding Project was delivered by Dr. Hitoshi Nakagawa (Project Leader of Japan), and followed by three special lectures by Japan and Korea as follows.

-Special Lecture 1: Title “Establishment of Ion Beam Technology”
( Dr. Atsushi Tanaka, JAEA, Japan)
-Special Lecture 2: Title “Mutation Breeding and Gene Isolation in Chrysanthemum”
(Dr. Geung Joo Lee, KAERI, Korea )
-Special Lecture 3: Title “High Amino Acid Accumulating Rice Mutants”
(Dr. Dong Sub Kim, KAERI, Korea)

Session 2   Country Report

Each participating country (Bangladesh, Indonesia, Japan, Malaysia, The Philippines, Thailand, Vietnam and Korea) presented their progress and general reports on Mutation Breeding in each country.

Attachment III: Country reports

Session 3   Sub-Project on Disease Resistance in Banana

The reports on Sub-project on Disease Resistance in Banana was presented by Dr. Masrizal (State ministry of Research and Technology, Indonesia) and Dr. Rusli Ibrahim (Malaysian Nuclear Agency, Malaysia). The progress Reports were not submitted from other participating countries (Bangladesh, the Philippines and Vietnam) because of miscommunication.

Attachment IV: Sub-project for banana

In the presentation, comments and recommendations are pointed as follows:

- The importance of screening for virus using ELISA was recommended especially for BBTV, but the test is expensive.
- The project on banana for Indonesia has already been terminated at the end of 2006 due to lack of budget.
- Currently, the project in Malaysia is in collaboration with a private company called Jalur Lipur Sdn. Bhd. which supported the field screening for Fusarium wilt disease and Malaysia will continue the project until 2008.
- A Special meeting of sub-project on disease resistance in banana in 2008 is proposed to be held either in Malaysia, Philippines or Japan. All the participating countries must submit the final report.
- Participating countries (Bangladesh, the Philippines and Vietnam) that did not submit progress reports during this workshop must send their reports to the secretariat (NSRA).

Session 4   Report Sub- Project Meeting on Insect Resistance in Orchid

The final report of Sub-Project Meeting on Insect Resistance in Orchid was presented by Assoc. Prof. Arunee Wongpiyasatid (Kasetsart University, Thailand)

Attachment V: Sub-project for orchid

Conclusion is as follows;
Due to the fact that there was no potential mutant obtained, therefore during the Sub-Project Meeting on Insect Resistance on Orchid which was held at Kasetsart University, Thailand in August 2007, FNCA proposed to extend the project for another 2 years until 2009.

Project Leaders from all the participating countries gave the following responses to the proposal of the Orchid Special Meeting:

Indonesia:   did not agree to extend the project.
Malaysia:   agreed to extend the project. Currently Nuclear Malaysia has a project with JAEA on the improvement of Orchid using ion beam.
Thailand:   did not agree to extend the project.

Even though Malaysia is the only participating country that agreed to continue with the project, all participants agreed to extend and support the project for another 2 years until 2009.

Session 5   Open Lecture 2 and Sub- Project Composition or Quality in Rice

Special lectures were delivered by Korea as follows.

-Special Lecture 4: Title “Rice genetic resources by mutation and their exploitation”
(Dr. Young Sub Shin, RDA, Korea )
-Special Lecture 5: Title “Analysis methods for functional compounds in rice“
(Prof. Young Sang Lee, Soonchunhyang Univ. Korea )

The reports on Sub-project on Composition or Quality in Rice was presented by all participating countries.

Attachment VI: Sub-project for Rice

After the discussion, the Agreement was made to proceed with the project.

Attachment VII: Agreement of Sub-Project on Rice

Session 6   Round Table Discussion

Duties and responsibilities of the Project Leaders,

- To collect all the progress reports from the Sub-Project Leaders
- To compile all the reports and send to the secretariat (NSRA) before the meeting
- To present the report during meeting if the Sub-Project Leader do not participate.
- The Project Leader, as a leader of mutation breeding researches in his or her country, will take part in coordinating and management of the Mutation Breeding Project and need to communicate with the Sub-Project Leader.

Minutes

The Minutes were discussed and agreed upon by all participants in the workshop. This will be reported at the Ninth Coordinators Meeting to be held in March 2008 in Tokyo, Japan

Technical Visit

Participants made a technical visit to Advanced Radiation Technology Institute of KAERI.

Attachments:

  1. Program
  2. Participants List
  3. Country Report
  4. Sub-project for banana
  5. Sub-project for orchid
  6. Sub-project for rice
  7. Agreement of Sub-Project on Rice

Program of
FNCA 2007 Workshop on Mutation Breeding

November 19 - 23, 2007
Daejeon Metropolitan City, Korea

Date : November 19-23, 2007
Venue : Nuclear Human Resource Development Center(NHRDC)
Korea Atomic Energy Research Institute (KAERI)
Daejeon Metropolitan City
Korea
Host
Organization :
Ministry of Education, Culture, Sport, Science and Technology(MEXT), Japan
Korea Atomic Energy Research Institute (KAERI)
Secretariat : Nuclear Safety Research Association (NSRA)
Working
Language :
English
 
Mon., 19 November
Opening Session
08:30 - 09:00 Registration
09:00 - 09:20 Opening Address: Dr. Sung Kee Cho (Director, Radiation Research Center for Bio-technology, ARTI-KAERI )
09:20 - 09:50 Group photo and Break
Session 1   Open Lecture 1
Chair : Japan
09:50 - 10:15 Project Review of FNCA Mutation Breeding Project ( Hitoshi Nakagawa, Project Leader of Japan )
10:15 - 10:45 Special Lecture 1: Title “Establishment of Ion Beam Technology” ( Dr. Atsushi Tanaka, JAEA, Japan)
10:45 - 11:15 Special Lecture 2: Title “Mutation Breeding and Gene Isolation in Chrysanthemum ” (Dr. Geung Joo Lee, KAERI, Korea )
11:15 - 11:45 Special Lecture 3: Title “High Amino Acid Accumulating Rice Mutants” ( Dr. Dong Sub Kim, KAERI, Korea)
11:45 - 13:00 Congratulation Reception (Lunch)
Session 2   Country Report
Chair : The Philippines
13:00 - 13:20 Bangladesh
13:20 - 13:40 Indonesia
13:40 - 14:00 Japan
14:00 - 14:20 Malaysia
14:20 - 14:40 The Philippines
14:40 - 15:00 Break
15:00 - 15:20 Thailand
15:20 - 15:40 Viet Nam
15:40 - 16:00 Korea
16:00 - 16:45 Discussion, Question and Answer
Tue., 20 November
Session 3   Sub-Project Disease Resistance in Banana
Chair : Thailand
09:00 - 09:30 Indonesia
09:30 - 10:00 Malaysia
10:00 - 10:30 Break
10:30 - 11:00 Discussion on Disease Resistance in Banana
Session 4   Report Sub- Project Meeting on Insect Resistance in Orchid
11:00 - 11:30 Report of Sub-Project Meeting on Insect Resistance in Orchid
(Assoc. Prof. Arunee Wongpiyasatid, Kasetsart University, Thailand )
11:30 - 12:00 Discussion on Insect Resistance in Orchid
12:00 - 13:00 Lunch
Cont. of Session 4   Report Sub- Project Meeting on Insect Resistance in Orchid
Chair : Japan
13:00 - 13:45 Discussion on Insect Resistance in Orchid (Cont.)
13:45 - 14:40 Discussion on Future Plan and Prospect of MB Project
14:40 - 15:00 Break
Session 5   Open Lecture 2 and Sub- Project Composition or Quality in Rice
Chair : Japan, Viet Nam
15:00 - 15:30 Special Lecture 4: Title “Rice genetic resources by mutation and their exploitation” (Dr. Young Sub Shin, RDA, Korea )
15:30 - 16:00 Special Lecture 5: Title “Analysis methods for functional compounds in rice “ (Prof. Young Sang Lee, Soonchunhyang Univ. Korea )
16:00 - 16:10 Break
16:10 - 16:35 Japan ( Dr. Minoru Nishimura, IRB, NIAS, Japan )
16:35 - 17:00 Discussion, Question and Answer
Wed., 21 November
Cont. of Session 5   Sub- Project Composition or Quality in Rice
Chair : Indonesia
09:00 - 09:30 Bangladesh
09:30 - 10:00 Indonesia
10:00 - 10:30 Korea
10:30 - 10:50 Break
10:50 - 11:20 Malaysia
11:20 - 11:50 Discussion, Question and Answer
11:50 - 13:00 Lunch
Cont. of Session 5   Sub- Project Composition or Quality in Rice
Chair : Korea
13:00 - 13:30 The Philippine
13:30 - 14:00 Thailand
14:00 - 14:30 Viet Nam
14:30 - 14:45 Break
14:45 - 15:45 Discussion about Working Plan on Rice Project
15:45 - 16:45 Discussion about Agreement on Rice Project
Thu., 22 November
Technical Visit to Advanced Radiation Technology Institute of KAERI
Fri., 23 November
Cont. of Session 5    Sub- Project Composition or Quality in Rice
Chair : Japan
09:00 - 10:30 Agreement on Rice Project
10:30 - 10:45 Break
10:45 - 11:45 Discussion about Working Plan on Rice Project
11:45 - 13:15 Lunch
13:15 - 14:30 Discussion about Working Plan on Rice Project
14:30 - 14:45 Break
Session 6   Round Table Discussion
Chair : Japan
14:45 - 16:00 Discussion on Future Plan and Prospect of MB Project
16:00 - 16:45 Drafting Minutes and Wrap up : Adoption of Minutes
16:45 - 17:00 Closing

List of Participants
FNCA 2007 Mutation Breeding Project Workshop

November 19 - 23, 2007
Daejeon, Korea


No.

Country

Name/Designation

1 Bangladesh Dr. Md. Lokman Hakim
Principal Scientific Officer
Institute of Food & Radiation Biology, Atomic Energy Research Establishment
Bangladesh Atomic Energy Commission (BAEC)
2 Bangladesh Mr. Golam Ahmed
Principal Scientific Officer
Plant Biotechnology and Genetic Engineering Division
Institute of Food and Radiation Biology
Bangladesh Atomic Energy Commission (BAEC)
3 Indonesia Dr. Masrizal
Assistant Deputy for Networking System of Science and Technology
The State Ministry of Research and Technology of Indonesia
4 Indonesia Dr. Sobrizal
Senior Research Officer
Center for the Application of Isotopes and Radiation Technology
National Nuclear Energy Agency (BATAN)
5 Japan Dr. Hitoshi Nakagawa
Director
Institute of Radiation Breeding
Division of Genome and Biodiversity Research
National Institute of Agrobiological Sciences (NIAS)
6 Japan Dr. Atsusi Tanaka
Unit Manager
Radiation-applied Biology Division
Quantum Beam Science Directorate
Japan Atomic Energy Agency (JAEA)
7 Japan Dr. Minoru Nishimura
Senior Officer
Institute of Radiation Breeding
Division of Genome and Biodiversity Research
National Institute of Agrobiological Sciences (NIAS)
8 Japan
(Secretariat)
Ms. Aki Koike
International Affairs and Research Department
Nuclear Safety Research Association (NSRA)
9 Korea Dr. Si-Yong Kang
Principal Researcher, Project Leader
Advanced Radiation Technology Institute (ARTI)
Korea Atomic Energy Research Institute (KAERI)
10

Korea
(Secretariat)

Ms. Kyungmee Yoo
Nuclear Human Resource Development Center(NHRDC)
Korea Atomic Energy Research Institute(KAERI)
11 Korea Dr. Geung-Joo Lee
Senior Researcher
Advanced Radiation Technology Institute (ARTI)
Korea Atomic Energy Research Institute (KAERI)
12 Korea Dr. Dong Sub Kim
Senior Researcher,
Advanced Radiation Technology Institute (ARTI)
Korea Atomic Energy Research Institute (KAERI)
13 Korea Dr. Young Sub Shin
Senior Researcher
Institute of Crop Science
Rural Development Administration (RDA), Korea
14 Korea Dr. Young Sang Lee
Prof., Soonchunhyang Univ. Korea
15 Malaysia Dr. Rusli Bin Ibrahim
Rice Breeder
Agrotechnology & Biosciences Division
Malaysian Nuclear Agency (Nuclear Malaysia)
16 Malaysia Dr. Abdul Rahim Harun
Senior Research Officer
Agrotechnology & Biosciences Division
Malaysian Nuclear Agency (Nuclear Malaysia)
17 The Philippines Ms. Faye G. Rivera
Science Research Specialist II
Philippine Nuclear Research Institute (PNRI)
18 Thailand Assoc. Prof. Arunee Wongpiyasatid
Associate Professor
Department of Radiation and IsotopesFaculty of ScienceKasetsart University (KU)
19 Thailand

Mr. Suniyom Taprab
Plant Breeder
Rice Department
Ministry of Agriculture and Cooperatives

20 Thailand

Ms. Dara Chettanachit
Senior Expert
Rice Department
Ministry of Agriculture and Cooperatives

21 Viet Nam

Dr. Mai Quang Vinh
Director
Centre For Consultancy and Technology Transfer in Agriculture, National Institute of Agricultural Genetics (AGI)

22 Viet Nam Dr. Le Xuan Tham
Deputy Director
Center for Nuclear Techniques Ho Chi Minh City
Vietnam Atomic Energy Commission (VAEC)

Agreement of Sub-Project on Rice


1. Agreement on the exchange breeding material under the framework of FNCA Mutation Breeding Project
General Rules and Regulations:
1) FNCA should be the coordinating agency for facilitating exchange program.
2) There must be Material Transfer Agreement (MTA) between two institutions involved
2. Request for use of irradiation such as Ion beam, Gamma Ray Chronic Irradiation for participating countries must be channelized through the Secretariat Office.
3. A standard variety/ methodology shall be used by all participating countries
Standard variety for both amylose and protein content (IR-64 for Indica rice and Koshihikari for Japonica rice).
Methodology:
1) Amylose content shall be determined using the IRRI method.
2) Protein content shall be determined by kjeldahl method.
3) Simple standardized method for preliminary screening of amylose content shall be used in M2 or M3 generations (Thailand or IRRI method).
4) A conventional SDS-PAGE method to analyze digestible and indigestible protein will be provided by IRB, Japan.

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